TY  - JOUR
T1  - Determination of Minimal Fractionation Period Required for
Extracting Humic Acid from Paddy Husk Compost
AU - Ywih Ch'ng, Huck AU - Aisyah, Fadzillah AU - Othman Osman, Suhaimi 
JO  - Research Journal of Applied Sciences
VL  - 13
IS  - 10
SP  - 626
EP  - 631
PY  - 2018
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2018.626.631
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2018.626.631
KW  - Paddy husk compost
KW  -fractionation period
KW  -humic acid
KW  -phenolic and carboxylic
KW  -total acidity
KW  -saturated
AB  - The process of isolating humic substances, especially, humic acid (extraction, fractionation and
purification) is time consuming, laborious and expensive. The objective of this study was to determine the
minimum period required to fractionate humic acid from paddy husk compost. The rice straw compost was
produced by mixing 80% of shredded rice straw, 10% of goat manure slurry, 5% of chicken feed and 5% of
molasses. During the extraction process, 10 g of compost was placed in the centrifuge bottle, added
with 100 mL of 0.5 M NaOH. The samples were equilibrated at room temperature for 24 h. After that, the mixture
was centrifuged at 10, 000 G for 15 min. During the fractionation process, the pH of the solution was adjusted
to 1.0 by using 6 M hydrochloric acid. Then the humic acid was allowed to equilibrate for 1, 3, 5, 7, 9, 12 and
24 h, respectively and the suspension was centrifuged. Purification was done subsequently to purify the humic
acid.The yield of humic acid fractionated under 1 h was 0.26 g and was not statistically different from 3, 5, 7, 9,
12 and 24 h. This indicates the fractionation period of 1 h also had the same time taken as 3, 5, 7, 9, 12 and
24 h for the exchange sites of humic acid of compost to be saturated with H+ions after acidification. Phenolic,
carboxylic and total acidity were determined and falls within the range reported by literatures. The optimum
fractionation period of humic acid from paddy husk compost was 1 h as the yield of humic acid under different
fractionation periods were not significantly different. Therefore, by using 1 h of fractionation period it will
absolutely save the consumption of chemicals, electricity and labor costs.
ER  -