TY  - JOUR
T1  - Evaluation of the use of Novel Coliphages to Control <I>Escherichia coli</I> W<SUB>1</SUB> and <I>Escherichia coli</I> W<SUB>2</SUB> Strains Isolated from 
  Water
AU - Enan, Gamal AU - Shaaban, Khalid A. AU - Askora, Ahmed AU - Maher, Muhammad 
JO  - Research Journal of Applied Sciences
VL  - 8
IS  - 10
SP  - 486
EP  - 493
PY  - 2013
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2013.486.493
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2013.486.493
KW  - Escherichia coli W1 and Escherichia coli W2 (E. coli W1 and E. coli W2)
KW  -. coli phages1-3 (ECP1-3)
KW  -electron microscopy
KW  -adsorption
KW  -biocontrol
AB  - This research described a preliminary studies for controlling 
  two <I>Escherichia coli</I> strains (<I>E. coli</I> W<SUB>1</SUB> and <I>E. 
  coli</I> W<SUB>2</SUB>) isolated from polluted water by their novel coliphages 
  which identified herein and designated ECP<SUB>1</SUB>, ECP<SUB>2</SUB> and 
  ECP<SUB>3</SUB>. <I>E. coli</I> W<SUB>1</SUB> and <I>E. coli</I> W<SUB>2</SUB> 
  strains were isolated from polluted water and identified using usual cultural, 
  Morphological and Biochemical Methods. Three bacteriophages lysing <I>E. coli</I> 
  strains were isolated. The bacteriophage lysing <I>E. coli</I> W<SUB>1</SUB> 
  (coliphage<SUB>1</SUB>) was designated ECP<SUB>1</SUB> while coliphage<SUB>2</SUB>, 
  coliphage<SUB>3</SUB> were highly specific to infect <I>E. coli</I> W<SUB>2</SUB> 
  and consequently have been designated ECP<SUB>2</SUB>, ECP<SUB>3</SUB>, respectively. 
  Transmission electron microscopy showed that the ECP<SUB>1</SUB>, ECP<SUB>2</SUB> 
  and ECP<SUB>3</SUB> coliphages belong to family Myoviridae. Increasing host 
  age lead to inhibition of coliphage infectivity. The ECP<SUB>1</SUB>, ECP<SUB>2</SUB> 
  and ECP<SUB>3</SUB> coliphages exhibited different rates of adsorption and burst 
  sizes but they possessed the same latent and rise periods (30 min). The host 
  range of the three coliphages suggested that these coliphages may be useful 
  as biocontrol agents. Addition of ECP<SUB>1</SUB>, ECP<SUB>2</SUB> and ECP<SUB>3</SUB> 
  coliphages to their hosts decreased viable cell population of their host <I>E. 
  coli</I> W<SUB>1</SUB>, W<SUB>2</SUB> distinctively <I>in vitro</I>.
ER  -