TY  - JOUR
T1  - Effect of Malathion on Biochemical Alterations in <I>Corcyra cephalonica</I>
AU - Ansari, Mohammad Ismail AU - Patel, Nisar G. AU - T.  Wankhedkar, Prakash 
JO  - Research Journal of Applied Sciences
VL  - 8
IS  - 2
SP  - 139
EP  - 142
PY  - 2013
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2013.139.142
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2013.139.142
KW  - Corcyra cephalonica
KW  -glucose
KW  -glycogen
KW  -protein
KW  -pesticide
KW  -malathion
AB  - The present research is carried out to observe the biochemical 
  effect of Malathion on the glucose, glycogen and protein levels. The effects 
  of LC<SUB>50</SUB> of commercial malathion (Hindustan Insecticide Ltd.) C<SUB>10</SUB>H<SUB>19</SUB>O<SUB>6</SUB>PS<SUB>2</SUB> 
  were tested in the laboratory on the larvae of rice moth <I>Corcyra cephalonica</I>. 
  The rice moth <I>Corcyra cephalonica </I>(Stainton) (Lepidoptera-Pyralidae) 
  is an important stored grain pest in Asia and South America. Its larval stages 
  causes serious damage to rice, gram, sorghum, maize, ground nut, cotton seeds, 
  peanuts, linseeds, raisins, nutmeg, chocolates, biscuits, wheat, coffee, cocoa, 
  beans and milled products. Already contaminated or damaged stored food grains 
  by <I>C. cephalonica</I> were collected from Dhule Dist. Maharashtra State, 
  India and were reared in the laboratory conditions. The standard culture of 
  this insect was maintained in the laboratory on normal dietary medium composed 
  of coarsely ground jowar (Sorghum vulgare) mixed with 5% (w/w) yeast powder 
  at 26&plusmn;1&deg;C and 93&plusmn;5% Relative Humidity (RH). Young Corcyra larvae hatched 
  out from the egg within 3-4 days and fed on the grains by webbing. The larval 
  period lasted about 20-25 days and pupation took place inside the web itself 
  moths start emerging after 35-40 days.The objective was to examine the changes 
  in carbohydrate (glucose and glycogen) and protein contents. The larvae were 
  released on treated as well as normal dietary medium. After 24 h 50% mortality 
  was found at 8 ppm dose level. Then, 1/5th of LC<SUB>50</SUB> concentration 
  were mixed with dietary medium and allowed larvae to feed for 168 h the surviving 
  larvae were taken for estimation of glucose, glycogen and protein. After treatment 
  lived larval bodies were used for estimation of carbohydrate (glucose and glycogen) 
  and protein content. Recovery changes were also noted in the remaining lived 
  larvae after 168 h of normal feeding. It was found that the concentration of 
  glucose in normal, treated and recovery samples were 900 &#956;g, 550 &#956;g 
  and 720 &#956;g mL<SUP>-1</SUP> , respectively while glycogen was 800 &#956;g, 
  500 &#956;g and 650 &#956;g mL<SUP>-1</SUP>, respectively and protein was 200 
  &#956;g, 320 &#956;g and 290 &#956;g mL<SUP>-1</SUP>, respectively.
ER  -