TY  - JOUR
T1  - Changes in Membrane Permeability During Rehydration of Freeze-Dried <I>Weissella paramesenteroides</I> LC11
AU - Thonart, P. AU - Dje, K.M. AU - Soro, A.A. 
JO  - Research Journal of Applied Sciences
VL  - 5
IS  - 1
SP  - 7
EP  - 12
PY  - 2010
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2010.7.12
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2010.7.12
KW  - lactic acid bacteria
KW  -freeze-drying
KW  -Viability
KW  -starter culture
KW  -membrane integrity
KW  -fermented foods
AB  - The objective of this study was to assess changes in membrane permeability during rehydration of freeze-dried <I>Weissella paramesenteroides</I> LC11. Viability was assessed using the electrical conductivity measurement (ms/cm/g dry weight) and the plate count method (cfu g<SUP>-1 </SUP>dry weight). The symptoms of injury included an increase in the electrolyte leakage during the first 4 h of rehydration in Milli Q water and a decrease in the survival rate, suggesting an increase in membrane permeability during dehydration. An increase in the temperature, NaCl or monosodium glutamate concentration and a decrease in H<SUP>+</SUP> concentration resulted in an increase in the electrolyte leakage and a decrease in the survival rate. However, a decrease in the electrolyte leakage was observed with increasing glycerol, sucrose or maltodextrin and resulted in the maintenance of cell viability. Regression analysis showed that overall, there was a good correlation between the survival rate and electrolyte leakage (R<SUP>2</SUP>&gt;0.90). Change in membrane permeability might lead to electrolyte leakage during rehydration and ultimately, cell death.
ER  -