TY  - JOUR
T1  - <I>In silico</I> Annotation of the Genes Involved in Biosynthesis of Lipopolysaccharide for <I>Burkholderia pseudomallei</I>
AU - Al-Haj, Nagi A. AU - Suang, Lai L. AU - Sekawi, Zamberi AU - Shamsudin, Mariana N. AU - Abdullah, Rasedee AU - Mohamed, Rahmah 
JO  - Research Journal of Applied Sciences
VL  - 4
IS  - 2
SP  - 62
EP  - 72
PY  - 2009
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2009.62.72
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2009.62.72
KW  - Melioidosis
KW  -Burkholderia pseudomallei
KW  -open reading frames
KW  -lipopolysaccharides
KW  -bioinformatics
KW  -data mining
KW  -genes
AB  - <I>Burkholderia pseudomallei</I> is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of <I>B. pseudomallei</I> infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study, by using the available <I>B. pseudomallei</I> genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in <I>Burkholderia pseudomallei</I>. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for <I>B. pseudomallei</I> and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen.
ER  -