TY  - JOUR
T1  - Profile of VEGF Secretion in Human Peripheral Blood Mononuclear Cells <I>In vitro</I>
AU - Hajighasemi, Fatemeh 
JO  - Research Journal of Biological Sciences
VL  - 8
IS  - 6
SP  - 210
EP  - 214
PY  - 2013
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2013.210.214
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2013.210.214
KW  - VEGF
KW  -profile
KW  -human
KW  -PBMCS
KW  -therapeutic
AB  - Peripheral Blood Mononuclear Cells (PBMCs) are key regulators of inflammation. Vascular Endothelial Growth Factor (VEGF) is a cytokine which is produced by a number of cells and has an important role in inflammation. In this study, the patterns of VEGF expression by human PBMCs were assessed <I>in vitro</I> using Phytoheamagglutinin (PHA), Phorbol Myristate Acetate (PMA) and Lypopolysaccharide (LPS) as VEGF inducers. Human PBMCs were cultured in complete RPMI-1640 medium. Then, the cells were seeded at a density of 10<SUP>6</SUP> cells mL<SUP>-1</SUP> and subsequently were incubated with PMA (0-25 ng mL<SUP>-1</SUP>), PHA (0-10 &#956;g mL<SUP>-1</SUP>) or LPS (0-4 &#956;g mL<SUP>-1</SUP>) for 48 h. The level of VEGF secreted in the cell culture supernatants was measured with enzyme-linked immunosorbent assay kits (R and D systems). Human PBMCs cultured without any stimulus, produced detectable amount of VEGF after 48 h incubation. PMA significantly reduced while PHA/LPS significantly increased VEGF production in human PBMCs dose-dependently. The results show that PMA, PHA and LPS have different effects on VEGF production by PBMCs.
ER  -