TY  - JOUR
T1  - Detection the Toxigenic Genes of <I>Vibrio cholerae</I> and <I>Staphylococcus 
  aureus</I>
AU - AL-Haj, Nagi A. AU - Shamsudin, Mariana Nor AU - Rahim, Raha Abdul AU - Halimaton, H. AU - Suang, Lai L. AU - Mashan, M. Nurmas I. AU - Sekawi, Zamberi 
JO  - Research Journal of Biological Sciences
VL  - 8
IS  - 5
SP  - 155
EP  - 160
PY  - 2013
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2013.155.160
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2013.155.160
KW  - Vibrio cholerae
KW  -Staphylococcus aureus
KW  -enterotoxin
KW  -polymerase chain reaction
KW  -dot blot
KW  -sequencing
AB  - <I>Vibrio cholerae</I> has caused severe outbreaks of cholera 
  worldwide with thousands of recorded deaths annually while the <I>Staphylococcus 
  aureus</I> is one of the most significant pathogens causing nosocomial and community-acquired 
  infections. Conventional detection methods for diagnosis of clinical samples, 
  water and food based on culture, microscopy and biochemical testing are limited 
  by the speed of detection, sensitivity and specificity, so it is necessary to 
  develop innovative molecular methods for the rapid detect the presence genes, 
  expression levels of the toxigenic and drug target genes in <I>S. aureus</I> 
  and <I>V. cholerae</I> using PCR, sequencing and membrane array. The genes studied 
  are SEA-SEJ (genes encoding <I>S. aureus</I> enterotoxins) <I>ace</I>, <I>zot</I>, 
  <I>ctxA</I>, <I>ctxB</I>, <I>toxR</I> (toxigenic genes of <I>V. cholerae</I>) 
  <I>Sav1017</I> and <I>AdaB</I> (protein synthesis and DNA synthesis genes in 
  <I>S. aureus</I>. These techniques were carried out step by step with primers 
  designing, PCR amplification, sequencing and detection of expression by membrane 
  array. These assays are extremely robust, sensitive, specific and economical 
  and can be adapted to different throughputs. Thus, a rapid, sensitive and reliable 
  technique for detecting toxigenic genes of <I>S. aureus</I> and <I>V. cholerae</I> 
  was suc-cessfully developed.
ER  -