TY  - JOUR
T1  - Phytochemical Analysis and Cytotoxic Activity of <I>Ferulago carduchorum</I>
AU - Khanavi, Mahnaz AU - Golfakhrabadi, Fereshteh AU - Ostad, Seyed Nasser AU - Hafizi, Mitra AU - Ardekani, Mohammad Reza Shams AU - Saeidnia, Soodabeh AU - Akbarzadeh, Tahmineh 
JO  - Research Journal of Biological Sciences
VL  - 8
IS  - 5
SP  - 138
EP  - 142
PY  - 2013
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2013.138.142
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2013.138.142
KW  - Ferulago carduchorum
KW  -cytotoxic activity
KW  -MTT assay
KW  -phytochemical analysis
KW  -fraction
AB  - <I>Ferulago carduchorum</I> Boiss and Hausskn (Apiaceae) is 
  an endemic plant in West of Iran and it are used in dairy and oil ghee as a 
  natural preservative. The aerial parts of <I>F. carduchorum</I> were extracted 
  by percolation method with MeOH/H<SUB>2</SUB>O (80/20) and the fractions were 
  provided respectively with hexane, ethyl acetate, methanol, methanol/H<SUB>2</SUB>O 
  (50/50). The fractions have been investigated their phytochemical screening 
  and the cytotoxic activity against the colon carcinoma (HT29), the breast ducal 
  carcinoma (T47D), the hepatocellular carcinoma (HepG2) and Swiss mouse embryo 
  fibroblast (NIH 3T3) cell lines by MTT (3-(4, 5-di methyl thiazol-2-yl)-2, 5-diphenyltetrazolium 
  bromide) assay. Phytochemical screening revealed the presence of phytosteroids, 
  flavonoids, coumarins and saponins. The cytotoxicity of ethyl acetate and hexane 
  fractions showed more efficacy than the other fractions on T47D and HepG2 cell 
  lines (IC<SUB>50</SUB>&lt;100 &#956;g mL<SUP>-1</SUP>) could be attributed to 
  their content of coumarins and phytosteroids. The hexane fraction was selected 
  for phytochemical study and suberosin (a coumarin) isolated from hexane fraction 
  as an active compound. The Selective Index (SI) value of ethyl acetate fraction 
  was lower in HepG2, T47D and HT29 cell lines than hexane fraction that indicated 
  the selective effect of ethyl acetate fraction on this cell lines.
ER  -