TY  - JOUR
T1  - Development of Cultivation Media for Polyhydroxyalkanoates Accumulation in Bacterial Cells Isolated from Cassava Pulp
AU - Chansatein, Onuma AU - Urairong, Hathairat AU - Rodtong, Sureelak 
JO  - Research Journal of Biological Sciences
VL  - 7
IS  - 1
SP  - 31
EP  - 37
PY  - 2012
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2012.31.37
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2012.31.37
KW  - Development
KW  -cultivation media
KW  -polyhydroxyalkanoates
KW  -accumulation
KW  -E. coli
KW  -Thailand
AB  - Polyhydroxyalkanoates (PHAs) are biopolymers efficiently used as biodegradable plastics to replace environmentally unfriendly petroleum-derived plastics. The polymers can be synthesized by a wide range of microorganisms. Bacteria accumulate PHAs under conditions of nutrient stress particularly nitrogen or phosphorus limitations. In this study, cultivation media were developed for detecting a number of bacteria isolated from cassava pulp for their PHA production capability by modifying media described by potential references. Both complex and minimal media were developed when cultured at 35&deg;C for 48 h. The isolates were tested for the presence of PHA accumulation by straining with 1% Nile blue A and observed under the fluorescence microscope at excitation wave length of 650 nm. Transmission Electron Microscope (TEM) was used for comfirmation of PHA granules accumulation in bacterial cells. <I>Alcaligenes eutrophus </I>(TISTR 1095) and <I>E. coli </I>(TISTR 527) were used as the positive and negative control of PHAs-producing strain, respectively. This is the first report for the suitable media for detecting of PHAs-producing bacteria isolated from cassava pulp.
ER  -