TY  - JOUR
T1  - Effects of the Crude Polysaccharides from <I>Poria cocos</I> on the Proliferation and Differentiation of 3T3-L1 Cells
AU - Wang, Xun AU - Liu, Haifeng AU - Zhao, Ling AU - Dong, Han AU - Ning, Chunyou AU - Li, Xuewei AU - Cui, Hengmin 
JO  - Research Journal of Biological Sciences
VL  - 6
IS  - 11
SP  - 597
EP  - 601
PY  - 2011
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2011.597.601
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2011.597.601
KW  - Poria cocos
KW  -polysaccharides
KW  -3T3-L1
KW  -proliferation
KW  -differentiation
KW  -China
AB  - To study the effects of crude polysaccharides from <I>Poria cocos</I> (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 &#956;g mL<SUP>-1</SUP>) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 &#956;g mL<SUP>-1</SUP>). As the results, the different concentration PCCP (50, 100 and 200 &#956;g mL<SUP>-1</SUP>) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p&gt;0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 &#956;g mL<SUP>-1</SUP>) (p&lt;0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 &#956;g mL<SUP>-1</SUP>) (p&gt;0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.
ER  -