TY  - JOUR
T1  - Cytotoxic Activity of <I>Lythrum salicaria </I>L.
AU - Khanavi, Mahnaz AU - Moshteh, Mana AU - Manayi, Azadeh AU - Reza Shams Ardekani, Mohamad AU - Vazirian, Mahdi AU - Ajani, Yousef AU - Nasser Ostad, Seyed 
JO  - Research Journal of Biological Sciences
VL  - 6
IS  - 2
SP  - 55
EP  - 57
PY  - 2011
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2011.55.57
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2011.55.57
KW  - Cytotoxic activity
KW  -Lythrum salicaria L.
KW  -MTT assay
KW  -fraction
KW  -chloroform
KW  -cell
KW  -Iran
AB  - The aerial parts of <I>Lythrum salicaria </I>L. from Lahijan in Gillan province have been collected in June 2009. Total extract were obtained by MeOH/H<SUB>2</SUB>O (80/20) and then partitioned between CHCl<SUB>3</SUB>, EtOAc and MeOH. These fractions and total extract have been investigated for in vitro cytotoxic activity against colon carcinoma (HT-29), lukemia (K562), breast ductal carcinoma (T47D) and Swiss mouse embryo fibroblast (NIH 3T3) cell lines by MTT assay (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyltetrazolium bromide). At each cell line doses of 50, 100, 200, 400 and 800 &#956;g mL<SUP>-1</SUP> in 1% (v/v) DMSO of all samples were tested. Ethyl acetate fraction against proliferation of T47D cell line has been represented high cytotoxic activity (IC<SUB>50</SUB>&lt;70). Chloroform fraction and total extract against proliferation of T47D cell line and chloroform fraction toward K562 cell line and total extract against HT-29 cell line have been demonstrated slightly cytotoxic activity (IC<SUB>50</SUB>&lt;200). The other fractions have been demonstrated no effective cytotoxic activity. IC<SUB>50</SUB> values confirmed that the growth and proliferation of T47D cells were most affected by ethyl acetate fraction.
ER  -