TY  - JOUR
T1  - Growth Characteristics and Halocin Production by a New Isolate, <I>Haloferax volcanii </I>KPS1 from Kovalam Solar Saltern (India)
AU - Kavitha, P. AU - Lipton, A.P. AU - Sarika, A.R. AU - Aishwarya, M.S. 
JO  - Research Journal of Biological Sciences
VL  - 6
IS  - 5
SP  - 257
EP  - 262
PY  - 2011
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2011.257.262
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2011.257.262
KW  - Haloferax volcanii KPS1
KW  -halocin KPS1
KW  -halophilic agar
KW  -culture conditions
KW  -enzymes
KW  -India
AB  - An archeon <I>Haloferax volcanii</I> KPS1 was isolated from Kovalam saltern, Kanyakumari, India. It produced halocin which was active against gram positive and gram negative bacteria viz., <I>Bacillus subtilis</I>,<I> Streptococcus mutans</I>,<I> Staphylococcus aureus</I>,<I> Escherichia coli</I> and <I>Pseudomonas aeruginosa</I>, respectively. The halobacteria <I>H. sodomens </I>was inhibited to the maximum by the halocin KPS1 of the <I>Haloferax volcanii</I> KPS1. The growth and halocin production was maximized when the organism was grown at a temperature of 40&deg;C at the pH of 7.0 and when supplemented with 25% w/v NaCl to the halophilic broth medium. The results of the stability studies indicated that the halocin KPS1 became thermolabile at the temperature &gt;80&deg;C and was stable over a wide range of pH from 3.0-9.0. A loss of activity of halocin KPS1 was detected when treated with proteolytic enzymes like proteinase K and trypsin indicating the proteinaceous nature of the antibacterial compound. The halocin produced by <I>Haloferax volcanii</I> KPS1 would provide a lead for developing antimicrobial drugs.
ER  -