TY  - JOUR
T1  - Determination of Total Phenolic and Flavonoid Contents of <I>Leonurus cardiaca</I> L. in Compare with Antioxidant Activity
AU - Jafari, Samineh AU - Moradi, Arman AU - Salaritabar, Ali AU - Hadjiakhoondi, Abbas AU - Khanavi, Mahnaz 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 7
SP  - 484
EP  - 487
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.484.487
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.484.487
KW  - Leonurus cardiaca L
KW  -antioxidant
KW  -phenolic content
KW  -flavonoid
KW  -methanolic-aqueous
KW  -fraction
KW  -hyperoside equivalents
AB  - The crude extract and 4 fractions of aerial parts of cultivated motherwort (<I>Leonurus cardiaca </I>L.) were investigated for their total phenolic and flavonoid contents and antioxidant activities. The phenolic and flavonoid contents were determined using Folin-Ciocalteu assay and proposed assay of pharmacopeia where as antioxidant activity of samples were measured by 2 tests: DPPH and FRAP assays. Buthanolic fraction showed the highest antioxidant activity with the FRAP value of 1.735&plusmn;0.07 &#956;mol g<SUP>-1</SUP> and DPPH inhibitory percentage of 57.76% &#956;g mL<SUP>-1</SUP> as well as the greatest phenolics content (48.37&plusmn;3.76 mg of gallic acid equivalents g<SUP>-1</SUP>). The highest total flavonoid content was revealed in methanolic-aqueous fraction (50.21&plusmn; 0.65 mg of hyperoside equivalents g<SUP>-1</SUP>). There was a direct correlation between total phenol and antioxidant activities which could introduce phenols as the main antioxidants of <I>L. cardiaca </I>L. extracts.
ER  -