TY  - JOUR
T1  - The Effect of Source and Level of Dietary Chromium Supplementation on Humoral Antibody Response and Blood Chemical Parameters in Hybrid Tilapia Fish (<I>Oreochromis niloticus </I>x <I>O. aureus</I>)
AU - Magzoub, M.B. AU - Al-Batshan, H.A. AU - Hussein, M.F. AU - Al-Mufarrej, S.I. AU - Al-Saiady, M.Y. 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 7
SP  - 821
EP  - 827
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.821.827
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.821.827
KW  - Chromium supplementation
KW  -tilapia fish
KW  -blood parameters
KW  -immune response
KW  -SRBC
AB  - This study was undertaken to determine the effect of dietary chromium supplementation on humoral antibody response and some blood constituents in hybrid tilapia fish (<I>Oreohromis niloticus x O. aureus</I>). Cr-yeast or Chromic Oxide (Cr<SUB>2</SUB>O<SUB>3</SUB>) were added to fish feed at the rates of 1 or 2 mg kg<SUP>-1</SUP>, while control fish received unsupplemented diet. Three hundred and forty fish (Avg. weight 37.8 g) were randomly assigned to 5 feeding regimens with two replicates each. Each replicate was reared separately. The fish were immunized with Sheep Red Blood Cells (SRBCs) at 0 day and re-immunized at 30 days of the experiment to determine primary and secondary humoral antibody responses, respectively. Blood samples were collected from 15 fish/replicate at 0 and 25 days to determine blood plasma constituents and at 5 days intervals during primary and secondary immunization up to 50 days to determine anti-SRBC antibody titers. The results shown decreased plasma glucose and increased plasma cholesterol concentrations at 25 days compared to 0 days in all groups. Increased triglyceride levels was noted in fish receiving Cr<SUB>2</SUB>O<SUB>3</SUB> (1 mg kg<SUP>-1</SUP>) and decreased albumin: globulin ratio in those receiving Cr yeast (2 mg kg<SUP>-1</SUP>) at 25 days (p<0.05). No significant changes were observed in total protein, albumin and total globulin concentrations due to either Cr treatment or feeding duration. Anti-SRBC antibody titers were significantly higher in Cr-supplemented groups versus control, with highest titers in fish receiving 2 mg kg<SUP>-1</SUP> Cr-yeast supplement during secondary immunization. These findings indicate that the inclusion of Cr in the feed significantly augments humoral antibody response of tilapia.
ER  -