TY  - JOUR
T1  - Detection the Antibacterial Effect of Seaweeds on <I>Staphylococcus aureus</I> DNA Repair Gene (<I>adaB</I>) and Cell Wall Protein Synthesis (<I>sav1017</I>) by Molecular Approaches
AU - Al-Haj, Nagi A. AU - Mashan, Nurmas I. AU - Shamsudin, Mariana N. AU - Mohamad, Habsah AU - Vairappan, Charles S. AU - Sekawi, Zamberi 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 6
SP  - 662
EP  - 667
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.662.667
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.662.667
KW  - Polymerase chain reaction
KW  -Staphylococcus aureus
KW  -seaweeds
KW  -antimicrobial activities
KW  -extraction
KW  -Malaysia
AB  - Polymerase Chain reaction amplification of DNA was performed to used to study the presence and effect of treated and untreated<I> Stapylococcus aureus</I> genes<I> sav1017</I> and <I>adaB</I> with marine seaweeds <I>Gracilaria changii</I> and <I>Euchema denticulatum</I>. From the sequencing analysis, the changes were detected in the gene sequence of <I>adaB</I> and <I>sav1017</I>, genes after treated with either <I>G. changii</I> or <I>E. denticulatum</I> extract, which involved the substitution of the nucleotide base pair and insertion or deletion of the purine or pyrimidine base. The novel of this study is the extract of <I>G. changii</I> and <I>E. denticulatum</I> interrupting the important function in MRSA and non-MRSA isolates so that this pathogen cannot survive longer than usual. This significant finding can be applied to a medical treatment whereby both of these extracts can be used as an alternative treatment for the infection of <I>S. aureus</I> especially to overcome drug resistance treatment problems in MRSA strains.
ER  -