TY  - JOUR
T1  - Isolation of<I> Salmonella enteritidis</I> Using Biochemical Tests and Diagnostic Potential of <I>SdfI </I>Amplified Gene
AU - Mirmomeni, M.H. AU - Naderi, S. AU - Colagar, A. Hosseinzadeh AU - Sisakhtnezhad, S. 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 6
SP  - 656
EP  - 661
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.656.661
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.656.661
KW  - Salmonella enteritidis
KW  -SdfI gene
KW  -PCR
KW  -biochemical test
AB  - <I>Salmonella enteritidis </I>is a food borne pathogen that affects the human health by infecting poultries. The aim of this study is to set up methods for identifying <I>S. enteritidis</I>, based on molecular properties of this bacterium. In this study, two control isolates of RTCC1623 and RTCC1624, obtained from the institute of Razi (Karaj, Iran) and 76 Kermanshah poultry derived samples were isolated using biochemical tests. Genomic DNA was extracted by universal Phenol-Chloroform method and PCR was performed via the specific primers of <I>SdfI-F</I> and <I>SdfI-R</I> of the <I>SdfI</I> (AF370707) gene. Amplified fragments of the 333 (<I>SdfI</I>) base pairs were observed in 66 of the total 76 <I>S. enteritidis</I> isolates. This study recommends that the identification of these pathogens by PCR method can be replaced with traditional bacteriological techniques. The PCR method is a rapid approach for recognizing and identifying the <I>S. enteritidis</I> infections in chicken products.
ER  -