TY  - JOUR
T1  - Cloning and Sequencing of Strawberry Chitinase Gene CHI2
AU - , Zhang Yong AU - , Tang Hao-ru AU - , Luo Ya AU - , Jiang Hao AU - , Hou Yanxia 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 5
SP  - 624
EP  - 628
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.624.628
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.624.628
KW  - Strawberry
KW  -chitinase gene
KW  -cloning
KW  -sequencing
KW  -amino acid
KW  -CHI2
AB  - Two genes fragments from cultivated strawberry (Fragaria x ananassa cv. Toyonaka and cv. Akihime) genomic DNA were isolated by PCR amplification. The study showed that these genes were 1575 bp in length and contained a 988 bp Open Reading Frome (ORF) and 2 extron and 1 intron. The homology of nucleotide and deduced amino acid were 99.05 and 98.56%, respectively. Sequecing revealed that the 2 genes encoded a class chitinase. BLAST analysis showed that the homology of deduced amino acid between strawberry and other species’s CHI2 gene were about 50%. The homology tree showed that it obviously distinguished from the others.
ER  -