TY  - JOUR
T1  - Increased Resistance of Erythrocytes to Hemolysis During Hypoxic Hypoxia: Mechanism and Effect
AU - , B.O. Ekpo AU - , I. Elekwa 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 3
SP  - 268
EP  - 271
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.268.271
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.268.271
KW  - Barocamera
KW  -erythrocyte
KW  -erythrogramme
KW  -hemolysis
KW  -hypoxia
AB  - The mechanism underlying the increased resistance of the red blood cell to hemolytic influences, occurring during hypoxic hypoxia was investigated in male albino rats. Hypoxia was induced by “raising” the animals to an imaginary height of 5,000 metres above sea level through 10 days. Metabolic studies were conducted using the 4th and 5th cell populations obtained by fractionation in sucrose solution concentration gradient and situated at the 18% and 14% sucrose concentration zones. The activities of hexokinase, (HK) lactate dehydrogenase (LDH) and glucose-6- phosphate dehydrogenase (G-6PD); and the concentrations of glucose and lactic acid were investigated. Hypoxia caused increased metabolic flux through glycolysis in the cell fractions as observed in elevated activities of HK and LDH, decrease in glucose level (p≤0.1), and increased concentration of lactic acid in the 3rd and 5th days of adaptation (p≤0.1). The activity of G-6PD was also increased in the periods. A tendency towards normalization of enzyme activities and concentration of metabolites was observed in the 10th day of hypoxia.
ER  -