TY  - JOUR
T1  - Oncolysis of Breast, Liver and Leukemia Cancer Cells Using Ethyl Acetate and Methanol Extracts of <I>Goniothalamus umbrosus</I>
AU - , Siddig Ibrahim Abdel-Wahab AU - , Ahmad Bustamam Abdul AU - , Syam Murali Mohan AU - , Adel Sharaf Al-Zubairi AU - , Manal Mohamed Elhassan 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 2
SP  - 209
EP  - 215
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.209.215
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.209.215
KW  - Goniothalamus umbrosus
KW  -cancer
KW  -apoptosis
KW  -MCF-7
KW  -CEM-ss
KW  -HT29
AB  - This current study, aims to investigate the ethyl acetate and methanol extracts of <I>Goniothalamus umbrosus</I> for their anticancer effects on several human cancer cells namely, the MCF-7 breast cancer, HT-29 colon cancer and CEM-ss leukemia cell lines using a 3 days MTT (3-(4 and 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide) assay. Morphological changes and probable mode of cancer cell death induced by bioactive <I>G. umbrosus</I> extract were examined. DNA laddering assay was performed to assess endo-nucleosomal fragmentation. The MTT assay results revealed that only the ethyl acetate extract has anticancer effects on human breast cancer cells (MCF-7). Half maximal Inhibitory Concentration (IC<SUB>50</SUB>) of the ethyl acetate extract was found to be 24.5&plusmn;0.12 &#956;g mL<SUP>-1</SUP>. Both inverted and fluorescence microscopic studies demonstrated that treated MCF-7 breast cancer cells using IC<SUB>50</SUB> of the extract displayed a number of typical morphological changes. Appearance of membrane blebs, DNA condensation and fragmentation are significant signs of apoptosis, were observed. The above findings suggested that the ethyl acetate extract of <I>Goniothalamus umbrosus</I> has potential therapeutic effect towards human breast cancer cells that requires further investigations in future.
ER  -