TY  - JOUR
T1  - Scrutiny on Significant Factors of Hemin Catalyzed Ag Nanoparticle as Biosensor for RNA
AU - Shokuhi Rad, A. 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 12
SP  - 1284
EP  - 1287
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.1284.1287
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.1284.1287
KW  - Sensitive detection
KW  -nanoparticle
KW  -RNA
KW  -biosensor
KW  -ammoniacal Ag ion
KW  -Iran
AB  - A responsive electrical detection technique of nucleic acids has been demonstrated on submicrogapped biosensor. This method contain immobilizing of Peptide Nucleic Acid (PNA) probes in the gap areas of a pair of interdigited microelectrodes and subsequently hybridizing with their complementary target RNA. After hybridization, hemin molecules were introduced into the RNA strand via zirconium-phosphate and zirconium-carbonate chemistries. The newly attached hemin molecules act as a catalyst to accelerate reducing ammoniacal Ag ion to form Ag nanoparticles, which span the gap of the interdigitated microelectrode. The conductance of the Ag nanoparticles directly correlated with the number of the hybridized RNA molecules. Nearly 1 fM sensitivity was achieved under optimal conditions.
ER  -