TY  - JOUR
T1  - Physiological Race and Genetic Diversity Determination of <I>Fusarium oxysporum</I> f.sp. <I>melonis</I> by Differential Hosts and Molecular Marker Rapd in Northern and Razavi Khorasan Provinces
AU - , Noushin Shafagh AU - , Mahrokh Falahati Rastegar AU - , Behrouz Jafarpour 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 7
SP  - 790
EP  - 793
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.790.793
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.790.793
KW  - Melon
KW  -fusarium wilt
KW  -Fusarium oxysporum f.sp. melonis
KW  -RAPD-PCR
KW  -polymorphism
AB  - Fusarium wilt of melon, caused by <I>Fusarium oxysporum</I> f.sp. <I>melonis</I> is one of the most important diseases of this crop, which annually causes tremendous losses. Because of the importance of this disease in Northern and Razavi Khorasan provinces, race identification and genetic diversity of the pathogen was investigated by using the differential standard hosts cultivars and molecular marker RAPD. Total 15 from 20 isolates which obtained from 52 fields located at different geographical regions were identified as pathogenic in pathogenicity test. In formae speciales test, seedling of melon, cucumber, watermelon and pea were used and typical symptoms of disease observed on melon plants only, therefore <I>F. oxysporum</I> f.sp. <I>melonis</I> was identified  as  the  cause  of  wilting  disease in melon. At race determination test, differential hosts were used and  the  existence  of  race  1  in  the regions was proved. The result of RAPD-PCR bands showed a polymorphism among population of this fungus. Cluster analysis showed that there are 7 genotypic groups in <I>Fusarium oxysporum</I> f.sp. <I>melonis</I> population which designated as A to G.
ER  -