TY  - JOUR
T1  - Complementary DNA Cloning and Immunologic Characterization of a New<I> Platanus orientalis </I>Pollen Allergen, Pla or 1.0101
AU - , Nazanin Pazouki AU - , Mojtaba Sankian AU - , Taher Nejadsattari AU - , Ramezan-Ali Khavari-Nejad AU - , Abdol-Reza Varasteh 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 12
SP  - 1419
EP  - 1425
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.1419.1425
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.1419.1425
KW  - Allergen characterization
KW  -cDNA cloning
KW  -cyclophilin
KW  -invertase inhibitors
KW  -MALDI-TOF-MS
KW  -Platanus orientalis
KW  -recombinant allergen
KW  -SDS -PAGE immunoblotting
AB  - Oriental Plane trees, as <I>Platanus orientalis</I>, are an important source of airborne allergens in cities of the southwest Asia and southeast Europe. Diagnosis of type I allergy essentially depends on the availability of defined allergens, which can be provided by recombinant technology. This study was aimed to investigate molecular properties of a <I>Platanus orientalis </I>pollen allergen, designated as Pla or 1.0101 (accession number EU296476), to produce its immunoreactive recombinant counterpart in <I>Escherichia coli</I>. Molecular characterization of the <I>Platanus orientalis </I>pollen allergen was performed using, cDNA cloning, expression of the recombinant allergen in <I>Escherichia coli</I> and IgE immunoblotting of recombinant allergen<I>. </I>The 18 kDa allergen (Pla or 1.0101) was identified as an important IgE-binding component of <I>Platanus orientalis </I>pollen. The  Pla  or  1.0101-specific cDNA  sequences  were  amplified,  using  specific  primers  based  on the N and C-terminal sequence of a Pla or 1.0101 homologue in <I>Platanus acerifolia</I>, Pla a 1. Sequencing corresponding Pla or 1.0101 cDNA revealed an open reading frame of 513 bp coding for 170 amino acid residues. The recombinant Pla or 1.0101 was produced by pET102/D-TOPO <I>E. coli</I> expression system. IgE-binding to the recombinant form of Pla or 1.0101 was proven by immunoblot and specific ELISA.
ER  -