TY  - JOUR
T1  - Development of a PCR Primer and a Marker Band for Detection of <I>E. coli</I> from Various Sources Based on Arbitrary Primer Set
AU - , Nagi A. AL-Haj AU - , Mariana N. Shamsudin AU - , Raha A. Rahim AU - , Zamri Ishak 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 11
SP  - 1327
EP  - 1332
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.1327.1332
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.1327.1332
KW  - Random Amplified Polymorphic DNA (RAPD)
KW  -uropathogenic E. coli (UPEC)
KW  -Neonatal Meningitis-associated E. coli (MNEC)
KW  -University Putra Malaysia (UPM)
KW  -primer
KW  -sequence
AB  - Although, PCR methods aimed on the detection of genes associated with the pathogenicity of <I>Escherichia coli</I> have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 <I>E. coli</I> isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called <I>secD</I> was designed to be used as PCR primer. <I>secD</I> primer pairs was highly specific to detect all isolates including <I>E. coli</I> O157: H7.
ER  -