TY  - JOUR
T1  - Comparison of Techniques to Determine the Ruminal and Post-Ruminal Protein Disappearance of Various Oilseed Meals
AU - , M. Danesh Mesgaran AU - , H. Jahani-Azizabadi AU - , A.R. Heravi Moussavi 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 9
SP  - 1028
EP  - 1033
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.1028.1033
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.1028.1033
KW  - Protein disappearance
KW  -3-step procedure
KW  -in vitro enzymatic procedure
KW  -oilseed meal
AB  - Ruminal, post-ruminal and total tract crude protein disappearance of cottonseed meal containing 105 (CSMH) or 65 (CSML) g oil per kg of dry matter (DM), soybean meal (SBM), rapeseed meal (RSM) and sunflower meal (SFM), originating from Iranian plant varieties, were measured using <I>in situ</I> mobile bag, three-step <I>in situ</I>/<I>in vitro</I> (3-step) and <I>in vitro</I> enzymatic procedures. For the <I>in situ</I> mobile bag technique, 4 Holstein steers (450±50 kg, body weight) fitted with ruminal fistulae and T-shaped intestinal cannulae were used. Three-step procedure was followed by rumen incubation of samples for 12 h and enzymatic incubation of ruminal undegradable samples. <I>In vitro</I> enzymatic technique was followed by 1 h borate- phosphate buffer incubation, then 4 h in protease solution as ruminal disappearance and enzymatic (pepsin and pancreatin) incubation of ruminal undegradable samples. A significant (p&lt;0.01) effect was found between the procedures to measure ruminal, post-ruminal and total tract CP disappearance. The protein disappearance of oilseed meals in the rumen and post-rumen determined <I>in vitro</I> enzymatic procedure tended to be higher than 3-step technique. Post-ruminal protein disappearance measured with <I>in vitro</I> procedure was higher (p&lt;0.01) than <I>in situ</I> mobile bag and 3-step techniques (<I>In vitro</I> =  0.75, <I>In situ</I> mobile bag = 0.74 and 3-step = 0.60; S.E.M = 0.023, respectively). There was a significant (p&lt;0.01) effect of feeds on ruminal, post-ruminal and total tract CP disappearance. The CSMH<SUB> </SUB>had a lower (p&lt;0.01) ruminal CP disappearance, while the SFM had higher value (0.58 and 0.91, respectively). The CSMH had lower ruminal CP disappearance compared with CSML (0.58 vs. 0.68, respectively). The CSML<SUB> </SUB>had a lower (p&lt;0.01) post-ruminal CP disappearance, while the SBM had higher value (0.59 and 0.81, respectively). In addition, our results indicated that when used to assess post-ruminal disappearance of oilseed meal protein, the 3-step method can underestimate the disappearance of protein.
ER  -