TY  - JOUR
T1  - Delipidation of a Pestivirus: Viral Inactivation and Vaccine Development in Large Animals
AU - , Bill E. Cham AU - , A.D. Shannon AU - , X. Gu AU - , A. Elliott 
JO  - Research Journal of Biological Sciences
VL  - 2
IS  - 7
SP  - 706
EP  - 712
PY  - 2007
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2007.706.712
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2007.706.712
KW  - Delipidation
KW  -virus inactivation
KW  -vaccine development
KW  -BVDV
KW  -lipid-associated virus
AB  - Lipid Associated Viruses (LAVs) including HIV, hepatitis B, C and SARS are amongst the most malevolent viruses. These and a variety of other viruses have an outer lipid envelope which maintains inserted viral peptides in the &quot;correct&quot; functional conformation and orientation. Most solvents disrupt the lipid envelope and destroy infectivity but these solvents also result in a loss of antigenicity. Specific organic solvents consisting of butanol and Diisopropyl Ether (DIPE) delipidate the whole virion rendering it non-infective, but antigenic. In large animals such as cattle, delipidation of large amounts of the pestivirus Bovine Viral Diarrhea Virus (BVDV) result in the inactivation of the virus shown by <I>in vitro</I> and <I>in vivo</I> testing. The inactivated BVDV preparation when used as a vaccine results in anti-E2 antibody production in all vaccinated animals. Delipidation of lipid-associated enveloped viruses with specific organic solvents has potential as the basis for development of vaccines.
ER  -