TY  - JOUR
T1  - Therapeutic Efficacy of Garlic (<I>Allium sativa</I>) Against Burn Wound  Infection by <I>Pseudomonas aeruginosa</I>
AU - , M. Arzanlou AU - , R. Arab AU - , R. Alaei 
JO  - Research Journal of Biological Sciences
VL  - 2
IS  - 6
SP  - 634
EP  - 638
PY  - 2007
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2007.634.638
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2007.634.638
KW  - Pseudomonas aerugnosa
KW  -burn infection
KW  -silver sulphadiazine
KW  -garlic extract
AB  - Treatment of multi-drug resistant <I>Pseudomonas aeruginosa</I>,<I> </I>which causes<I> </I>burn infection is a big challenge in clinics and needs novel strategies. Garlic extract has potent <I>in vitro</I> antibacterial activities against number of gram negative and gram positive bacteria including<I> P. aeruginosa</I>. The aim of this study, was to evaluate <I>in vivo</I> therapeutic efficacy of garlic extract in treating burn wound infection caused by <I>P. aeruginosa</I> in burned mouse model. Burn was induced on the back of anesthetized animals by hot water, after the hair removal. Bacterial infection  was  established  by  topical  applying  of  highly  pathogenic  clinical isolate of <I>P. aeruginosa</I>.  Potential of garlic extract on reduction of mortality was evaluated by topical application of 10% (v/v) garlic extract on burned and infected animals (treatment group 1) and was compared with two control groups: Burned and  infected animals either treated with topical Silver Sulphadiazine (SSD) (1%) (treatment group 2) or left untreated (treatment group 3). The same groups were subjected to evaluate bacterial counts in organs (blood, liver, spleen and skin). Our results indicated that topical administration of garlic extract (10%) extended the survival of mice for 3-6 days, compared with survival of the untreated group. Both garlic extract and SSD treatments reduced the microbial loads in vital organs (blood, liver, spleen), compared to that of untreated control group (p< 0.05). These results demonstrate the potential of garlic extract in the treatment of burn wound infection caused by <I>P. aeruginosa</I>.
ER  -