TY  - JOUR
T1  - Detection and Identification of <I>Cryptosporidium</I> species in Water Samples from a River in Ardabil City, Northwestern Iran
AU - , Behnam Mohammadi Ghalebin AU - , Esmaeel Fallah AU - , Mohammad Asgharzadh AU - , Abdol Hassan Kazemi AU - , Mohsen Arzanlou 
JO  - Research Journal of Biological Sciences
VL  - 2
IS  - 4
SP  - 498
EP  - 502
PY  - 2007
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2007.498.502
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2007.498.502
KW  - Cryptosporidium species
KW  -PCR-RFLP
KW  -water
KW  -detection
KW  -indentification
KW  -Iran
AB  - <I>Cryptosporidium</I> is an opportunistic parasite typically associated with large waterborne outbreaks. Surface waters contaminated with human and animal feces serve as main source for epidemic spread of <I>Cryptosporidium </I>parasites. In this study, we used a small-subunit rRNA-based PCR-Restriction Fragment Length Polymorphism (RFLP) technique to determine the prevalence and to characterize human-infective species of <I>Cryptosporidium</I> parasites in water samples collected from a stream in Ardabil city in Iran. Among 30 samples examined, 11 samples showed positive results. Restriction pattern analysis showed <I>C. andersony</I> as the most common species with 7 cases; followed by <I>C. parvum</I>, bovine genotype, with 3 cases and <I>C. suis</I> with 1 case. The results indicated that PCR-RFLP technique provides an applicable and feasible method for detection and identification of <I>Cryptosporidium </I>oocysts in environmental water samples. The results, furthermore, demonstrated that wildlife is the major source of <I>Cryptosporidium </I>oocysts in surface water resources in the study region.
ER  -