TY  - JOUR
T1  - The Use of Oxolinic Acid to Enrich <I>Artemia urmiana</I> from Urmia Lake, Iran
AU - , Miryosef Yahyazadeh AU - , Amin Eimanifar AU - , William N. Camargo AU - , Mehdi Soltani AU - , Feridon Mohebbi 
JO  - Research Journal of Biological Sciences
VL  - 2
IS  - 3
SP  - 221
EP  - 224
PY  - 2007
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2007.221.224
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2007.221.224
KW  - Artemia urmiana
KW  -oxolinic acid
KW  -enrichment dosage and period
AB  - The live food enrichment technique using emulsions for preventing fish diseases was investigated as  a  tool  for  transferring  therapeutics  through  the  food chain. Nauplii and adult of the brine shrimp, <I>Artemia urmiana</I> were enriched with Oxolinic Acid (OXA) as an antibacterial drug to determine its content after different enrichment intervals. Various concentrations of OXA ranging from 25, 50, 75 and 100 mg L <SUP>1</SUP> were mixed with emulsion oil. Enriched nauplii and adult were sampled at 2, 4, 6, 8 h intervals. An analytical method for the quantification of OXA in a biological matrix, <I>A. urmiana</I> nauplii and adult, was developed using High Performance Liquid Chromatography (HPLC). The highest and lowest accumulation rate of OXA in enriched nauplii body tissue occurred at 100 and 75 mg L <SUP>1</SUP> at intervals 6 and 8 h (respectively) and for adult at was 50 and 25 mg L <SUP>1</SUP> at 4 and 8 h enrichment period (respectively). The average amounts of this were estimated to be 39  and  2.5  µg  g <SUP>1</SUP><I> </I> WW  (respectively)  for  nauplii  and  192  and  3  µg  g <SUP>1</SUP><I> </I>WW (respectively) for adult of <I>A. urmiana</I>. The obtained results showed that there is a significant outcome between enrichment dosage and period with content of OXA in both <I>A. urmiana</I> nauplii and adult (p< 0.001). However, a direct correlation could not be observed between enrichment dosage and period with OXA level in all enriched groups.
ER  -