TY  - JOUR
T1  - Susceptibility Faecal Isolates of Commensal <I>E. coli </I>from Cattle to Fluoroquinolones and Cephalosporins
AU - Ajayi, A.O. AU - Oluduro, O. AU - Olowe, O.A. AU - Famurewa, O. 
JO  - International Journal of Molecular Medicine and Advance Sciences
VL  - 6
IS  - 3
SP  - 43
EP  - 48
PY  - 2010
DA  - 2001/08/19
SN  - 1813-176x
DO  - ijmmas.2010.43.48
UR  - https://makhillpublications.co/view-article.php?doi=ijmmas.2010.43.48
KW  - Cephalosporins
KW  -animal medicine
KW  -regulated plasmids
KW  -spectrum
KW  -beta-lactamase
KW  -Nigeria
AB  - About 500 isolates of <I>Escherichia coli </I>were recovered from apparently healthy cattle and tested for their susceptibility to third generation cephalosporins and extended spectrum beta-lactamase producers were detected using the double dusk synergy test. Representative isolates were selected on the basis of their cephalosporin resistance patterns for plasmids analysis and mating experiment using <I>E. coli </I>25922 as recipient. Resistance to cefoxitin was highest as 395 (79.0%) showed resistance to the antibiotic while resistance to ceftriaxone was the lowest with 87 (17.4%) isolates. Seventy two isolates were confirmed to be extended spectrum beta-lactamase producers. Two of 13 representative isolates selected among the extended spectrum beta lactamase producers carried single plasmids while the remaining 11 isolates contain multiple plasmids ranging from 1.2-22.2 kb. The cephalosporin-resistant isolates also carried plasmids with sizes ranging from 4.32-32 kb. Twelve isolates among the extended spectrum beta-lactamase producers that carried plasmids successfully transferred the plasmids to the recipient while all the cephalosporin-resistant isolates successful transferred plasmids to the recipient strain. The use of third-generation cephalosporins in animal medicine should be regulated.
ER  -