TY  - JOUR
T1  - Survey and Determination of Mycotoxins in Wheat from Iran by LC/Fluorescence 
  Using Immunoaffinity Column Cleanup
AU - Feizy, Javad AU - Beheshti, Hamed Reza AU - Eftekhari, Zeynab 
JO  - Journal of Food Technology
VL  - 12
IS  - 1
SP  - 7
EP  - 10
PY  - 2014
DA  - 2001/08/19
SN  - 1684-8462
DO  - jftech.2014.7.10
UR  - https://makhillpublications.co/view-article.php?doi=jftech.2014.7.10
KW  - HPLC
KW  -aflatoxin
KW  -ochratoxin A
KW  -zearalenone
KW  -wheat
AB  - Mycotoxins are small, toxic chemical products formed as secondary 
  metabolites by a few fungal spices that readily colonise crops and contaminate 
  them with toxins in the field or after harvest. In this study, a Liquid Chromatographic 
  Method was developed for the simultaneous determination of Aflatoxins (AFs) 
  (B<SUB>1</SUB>, B<SUB>2</SUB>, G<SUB>1</SUB>, G<SUB>2</SUB>), Ochratoxin A (OTA) 
  and Zearalenone (ZEA) toxins in wheat. For this purpose, a total of 231 wheat 
  samples were analyzed. Mycotoxins were extracted and purified from the samples 
  using Immunoaffinity (IAC) columns. The 2.67 and 2.21% of examined wheat samples 
  contained AFB<SUB>1</SUB> and OTA. The method was based on the extraction of 
  AFs, OTA and ZEA finely ground wheat sample with 80% methanol and 60% acetonitrile 
  solution, respectively. The 2.60 and 2.16% of examined wheat samples contained 
  AFB<SUB>1</SUB> and OTA. LOD was 0.27, 0.04, 0.23, 0.04, 1.18 and 21.56 for 
  AFB<SUB>1</SUB>, AFB<SUB>2</SUB>, AFG<SUB>1</SUB>, AFG<SUB>2</SUB>, OTA and 
  ZEA, respectively. Considering these results these special commodities should 
  not be a health concern for these collectives, however, special attention should 
  be focused on imported foods distributed in special retail shops.
ER  -