TY  - JOUR
T1  - PCR Primers Designed from the UidA Gene Sequence for the Detection of Escherichia coli O157:H7
AU - , Moushumi Ghosh AU - , R. Son AU - , A. M. Sahilah AU - , K. Sushil AU - , W. L. Ooi AU - , I. Salmah AU - , S. H. Lim AU - , Sunita Bansal AU - , Abhijit Ganguli 
JO  - Journal of Food Technology
VL  - 2
IS  - 1
SP  - 59
EP  - 62
PY  - 2004
DA  - 2001/08/19
SN  - 1684-8462
DO  - jftech.2004.59.62
UR  - https://makhillpublications.co/view-article.php?doi=jftech.2004.59.62
KW  - 
AB  - One of the uniqueness of Escherichia coli O157:H7 is it inability to ferment sorbitol in 24 h and its negative test with the MUG assay, although this organism carried the UidA gene which encodes for -glucuronidase in its chromosome. Primers were designed based on the sequence of the -glucuronidase gene and were evaluated in a polymerase chain reaction assays as a marker to detect E. coli O157:H7. Of the three pairs of primers tested, which produces the estimated product size of 352, 271 and 353 bp respectively, one primer pairs (UidA2F & UidA2R) was found to be more specific and useful as a marker in combination with a published primer for the H7 gene for the detection of Escherichia coli O157:H7.
ER  -