TY  - JOUR
T1  - Expression of Ets Transcription Factor Etv5 Subfamily in Mouse Testis
AU - Ming Zhang, Xue- AU - Zhu, Wen-Qain AU - Si, Lei 
JO  - Journal of Animal and Veterinary Advances
VL  - 19
IS  - 8
SP  - 113
EP  - 116
PY  - 2020
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2020.113.116
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2020.113.116
KW  - Ets transcription factor
KW  -Etv5 subfamily
KW  -mouse
KW  -real-time PCR
KW  -testis
AB  - Spermatogenesis is a complicated biological
process which involves the comprehensive regulation of
the hormones, growth factors and transcription factors.
It’s demonstrated that the E26 transformation-specific
(Ets) family transcription factor Etv5 (also named as Ets
Related Molecule, ERM) plays a critical role in the
maintenance of male germline stem cell niche. However,
the basic expression levels of Etv5 and its peer member
Etv1 (also known as ER81) and Etv4 (also known as
Pea3) during the postnatal development and growth of
the testis are still unclear. To know this, testicular
samples were collected from 5-days-, 7, 16-weeks-and
10-months-old mice. Etv1, Etv4 and Etv5 were cloned
using reverse transcription PCR (RT-PCR). Real-time
PCR analysis showed that Etv1 remained higher
levels at 7-weeks and 10-months stages compared with
those of 5-days and 16-weeks stages (p<0.05,
respectively). Etv4 expression was very weak at all four
stages but also showing an increase tendency at 7-weeks.
Etv5 expression was lower at 5-days, extremely
increased at 7-weeks (p<0.01) and then dramatically
decreased at 16-week and 10-months stages (p<0.01).
Collectively, the mRNA expressions of Etv1 and Etv5 in
mouse testis reach peak along with the sexual maturity,
then decrease and maintain at a relatively high level
which putatively suggests Etv1 may has similar function
like Etv5 in spermatogenesis.
ER  -