TY  - JOUR
T1  - Characterization of Coagulase Negative Staphylococci Isolated from Dairy Goats in Germany
AU - Vazquez, Castañeda- AU - Sobiraj, A. AU - Ehrenberg, A. AU - Zschöck, M. AU - Castañeda Vazquez, M.A. AU - El-Sayed, A. 
JO  - Journal of Animal and Veterinary Advances
VL  - 19
IS  - 1
SP  - 1
EP  - 10
PY  - 2020
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2020.1.10
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2020.1.10
KW  - Germany
KW  -transfer DNA intergenic spacer length polymorphism PCR
KW  -coagulase negative Staphylococci
KW  -goat
KW  -NAS
KW  -subclinical mastitis
AB  - Over 2 years as a part of a surveillance
program, 2038 one half milk samples were collected
from dairy goats representing 12 German herds.
Microbiological investigation of the samples revealed
positive bacterial growth in 17.3% of them. Biochemical
identification of the field isolates detected Non-Aureus
Staphylococci (NAS) in 10.4%, Corynebacteria in 3.2%,
Streptococci in 0.8%, <i>S. aureus</i> in 0.7%, other bacteria in
1.0%. Meanwhile, mixed bacterial growth was shown in
2.2% of the samples. To identify the NAS isolates at their
species level the detection of their biochemical properties
alone using a commercial test kit enabled the correct
identification of a randomized selection of the field
isolates only in 11.4%. For further identification, a
combination of two other identification techniques
(antibiotic resistance profiles and molecular assays) was
additionally applied. By doing so, it was possible to
identify 69 (58.5%) out of 118 isolated presumptive NAS
field strains at their species level. The detected species
included <i>S. caprae</i>, <i>S. epidermidis</i>, <i>S. simulans</i>, <i>S. hyicus</i>,
<i>S. saprophyticus</i>, <i>S. chromogenes</i>, <i>S. lentus</i>, <i>S. xylosus</i> and <i>S. hominis</i>. Among them, <i>S. caprae</i> was detected
most frequently while <i>S. lentus</i>, <i>S. xylosus</i> and <i>S.
hominis</i> were isolated only from sporadic cases.
ER  -