TY  - JOUR
T1  - Pathogenicity of Fowl Adenovirus Seroype 8B Isolates of Malaysia in
Specific Pathogen Free Chickens
AU - Hair-Bejo, M. AU - Norfitriah, M.S. AU - Majdi, A. 
JO  - Journal of Animal and Veterinary Advances
VL  - 18
IS  - 3
SP  - 78
EP  - 83
PY  - 2019
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2019.78.83
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2019.78.83
KW  - Fowl Adenovirus (FAdV)
KW  -Inclusion Body Hepatitis (IBH)
KW  -Specific Pathogen Free (SPF) chicken
KW  -pathogenic
KW  -basophilic Intranuclear Inclusion Bodies (INIB)
KW  -UPM11134
AB  - Highly pathogenic Fowl Adenovirus (FAdV) is a causative agent of Inclusion Body Hepatitis (IBH)
in poultry. It causes mortality and poor performance of the affected chickens. It was objectives of the study to
determine pathogenicity of FAdV serotype 8b isolates of Malaysia in Specific Pathogen Free (SPF) chickens.
The virus isolates namely, UPM11134 and UPM11142 were obtained from IBH outbreaks in broiler chicken farms
and characterized as FAdV serotype 8b. The liver samples of affected chickens were prepared and inoculated
into SPF embryonated chicken eggs via. Chorioallantoic Membrane (CAM) route. The liver of embryos was
subsequently harvested for preparation of virus inoculum. The 36 days old SPF chicks were divided
into 3 groups namely groups A-C. Each group was further divided into sacrificed and mortality groups.
Chickens in groups A and B were inoculated with UPM11134 and UPM11142 FAdV isolates, respectively, via.
intraperitoneal route at day old. All chicken in group C remained uninoculated and acted as the control group.
The chicken were monitored for any clinical abnormalities throughout the trial. Gross lesions were recorded on
necropsy and samples of liver and gizzard were collected and fixed in 10% buffered formalin for histological
examination. The study showed 100 and 91% mortality of SPF chickens in groups A and B, respectively,
at day 4 post inoculation (pi). Clinical signs of IBH such as depression, weakness, ruffled feathers and
diarrhea were recorded within 12-24 h prior to death. On necropsy, hydropericadium with pale, friable and
petechial haemorrhages of liver were recorded. Hepatitis with areas of hepatic necrosis and haemorrhages and
presence of numerous basophilic Intranuclear Inclusion Bodies (INIB) in degenerated hepatocytes were
recorded in all chickens in groups A and B, whilst INIB were also observed in glandular epithelium of gizzard
in group A. All chicks in group C remained normal throughout the trial. It was concluded that Malaysian FAdV
serotype 8b isolates are highly pathogenic in SPF chickens and acted as the primary agent of IBH.
ER  -