TY  - JOUR
T1  - The Lengths of attI Sites Have No Effect on the Transcription and Expression of the Cassette and Integrase Genes
AU - Zheng, Fuying AU - Gong, Xiaowei AU - Chen, Qiwei AU - Liu, Yongsheng 
JO  - Journal of Animal and Veterinary Advances
VL  - 14
IS  - 3
SP  - 63
EP  - 68
PY  - 2015
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2015.63.68
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2015.63.68
KW  - transcription and expression
KW  -attI site
KW  -Class 1 integron
KW  -gene cassette
KW  -integrase gene
AB  - Class 1 integron is a genetic element with the ability to capture and express of the exogenous drug-resistance genes embedded in gene cassettes, offering bacteria with multi-drug resistance. The structures of the integron itself can regulate the transcription and express levels of the cassette genes. In this study, the effects of the attI sites on the transcription and express of the cassette and integrase genes were reported. pACYC184 plasmids, containing cloned integron fragments which differed only with respect to the lengths of attI sites were transferred into <I>E. coli</I> JM109. Then, the transcription and expression levels of the integrase and the first cassette gene after Pc promoter were detected in the corresponding recombinant strains. The results showed that the lengths of attI sites had no effect on the transcription and expression of the gene cassettes and integrase genes. This research was reported for the first time.
ER  -