TY  - JOUR
T1  - Biofilm-Producing <I>Staphylococcus aureus</I> Screening in Poultry Farms and Abattoirs
AU - Erfan, A.M. AU - Marouf, Sh. 
JO  - Journal of Animal and Veterinary Advances
VL  - 14
IS  - 10
SP  - 308
EP  - 314
PY  - 2015
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2015.308.314
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2015.308.314
KW  - S. aureus
KW  -poultry
KW  -biofilm
KW  -abattoirs
KW  -PCR
KW  -sequence
KW  -MTP
KW  -CRA
AB  - Surveillance on<I> Staphylococcus aureus</I> in poultry farms and poultry abattoirs in 2015 in Egypt was applied for determining the biofilm formation, an important virulence and antibiotic resistance determinant. Sixty-seven (68.3%) out of 98 poultry and environmental samples collected in this study were positive for <I>S. aureus</I>. For phenotypic analysis of biofilm formation, Microtitre Plate (MTP) and Congo Red Agar (CRA) tests revealed 63 (94.02%) and 59 (88.05%) positive samples, respectively. A high correlation (94.02%) between MTP and CRA tests was encountered. The PCR showed variable results for genes encoding Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) and for those encoding Polysaccharide Intercellular Adhesion/Poly-N-Succinyl-&#946;-1-6-Glucosamine (PIA/PNSG). Partial DNA sequencing of 606 nucleotides of the <I>icaR-icaA</I> fragment in 21 selected strains showed 98.2%-100% identity with two clearly distinct phylogroups. This study indicated that biofilm producing <I>S. aureus</I> are widely distributed in poultry and poultry abattoirs in Egypt.
ER  -