TY  - JOUR
T1  - Expression of Recombinant Baculovirus Vetor Double-expressing HA1 Gene from H5 Subtype Avian Influenza Virus and Mature Chicken Interleukin-18 Gene
AU - Kong, Na 
JO  - Journal of Animal and Veterinary Advances
VL  - 14
IS  - 5
SP  - 136
EP  - 139
PY  - 2015
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2015.136.139
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2015.136.139
KW  - Avian influenza virus
KW  -HA1 gene
KW  -mature ChIL-18 gene
KW  -eukaryotic expression
KW  -IL18 protein
AB  - The Hemagglutinin (<I>HA1</I>) gene from H5 subtype Avian Influenza Virus (AIV) and mature Chicken Interleukin-18 (<I>mChIL-18</I>) gene were amplified by designing two pairs of primers according to the sequence published on GenBank. The <I>HA1</I> gene and <I>mChIL-18</I> gene were inserted into the downstreams of PH promoter and P10 promoter of baculovirus expression plasmid pFastBac<SUP>TM</SUP>Dual, respectively. The recombinant pFastBac<SUP>TM</SUP>dual-mChIL-18-HA1 (rIL18-HA1) was ransformed into DH10Bac competent cells and expressed in sf9 cells by IFA diction. The results showed that the recombinant protein IL18-HA1 (pIL18-HA1) was simultaneouly expressed in the same sf9 cells with IL18 protein and HA1 protein, respectively. It is the foundation for developing a neotype vaccine against AIV.
ER  -