TY  - JOUR
T1  - Conserved Gene Structure and Function of Interleukin-10 in Teleost Fish
AU - Nam, Bo-Hye AU - Moon, Ji-Young AU - Park, Eun-Hee AU - Kim, Young-Ok AU - Kim, Dong-Gyun AU - Kong, Hee Jeong AU - Kim, Woo-Jin AU - Jung, Hyungtaek AU - Jee, Young Ju AU - Lee, Sang-Jun AU - An, Cheul min 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 12
SP  - 774
EP  - 782
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.774.782
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.774.782
KW  - Interleukin-10
KW  -genomic organization
KW  -promoter
KW  -mRNA expression analysis
KW  -Paralichthys olivaceus
AB  - Interleukin-10 (IL-10) is an important immunoregulatory cytokine 
  produced by various types of cells. Researchers describe here the isolation 
  and characterization of olive flounder IL-10 (ofIL-10) cDNA and genomic organization. 
  The <I>ofIL-10</I> gene encodes a 187 amino acid protein and is composed of 
  a five exon/four intron structure, similar to other known <I>IL-10</I> genes. 
  The ofIL-10 promoter sequence analysis shows a high level of homology in putative 
  binding sites for transcription factors which are sufficient for transcriptional 
  regulation ofIL-10. Important structural residues are maintained in the ofIL-10 
  protein including the four cysteines responsible for the two intra-chain disulfide 
  bridges reported for human IL-10 and two extra cysteine residues that exist 
  only in fish species. The phylogenetic analysis clustered ofIL-10 with other 
  fish IL-10s and apart from mammalian IL-10 molecules. Quantitative real-time 
  Polymerase Chain Reaction (PCR) analysis demonstrated ubiquitous <I>ofIL-10</I> 
  gene expression in the 13 tissues examined. Additionally, the induction of <I>ofIL-10</I> 
  gene expression was observed in the kidney tissue from olive flounder infected 
  with bacteria (<I>Edawardsiella tarda</I>) or virus (Viral Hemorrhagic Septicemia 
  Virus; VHSV). These data indicate that IL-10 is an important immune regulator 
  that is conserved strictly genomic organization and function during the evolution 
  of vertebrate immunity.
ER  -