TY  - JOUR
T1  - Effect of Dietary Vitamin E Supplementation on Oxidative Stress Response of Acute Nitrite Exposure in Sea Cucumber (<I>Apostichopus japonicus </I>Selenka) Juveniles
AU - Wang, Lei AU - Luo, Zuoyong AU - Wang, Baojie AU - Liu, Mei AU - Jiang, Keyong 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 2
SP  - 77
EP  - 88
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.77.88
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.77.88
KW  - Sea cucumber (Apostichopus japonicus Selenka)
KW  -vitamin E
KW  -nitrite stress
KW  -antioxidant enzyme
KW  -Reactive Oxygen Species (ROS)
KW  -Response Surface Methodology (RSM)
AB  - Different amounts of vitamin E supplementation were added 
  to feed sea cucumber (<I>Apostichopus japonicus</I> Selenka) juveniles (2.5&plusmn;0.15 
  g) testing to reduce nitrite stress. Commercial feed was used as the control 
  diet, 3 experimental diets containing vitamin E supplementation (150, 250 and 
  350 mg kg<SUP>-1</SUP> diet), respectively was designed to experiment for 45 
  days. The specimens were exposed to three different concentrations (0.5, 1.0 
  and 1.5 mg L<SUP>-1</SUP>) of nitrite for 4, 8 and 12 h at four different time 
  points (0, 15, 30 and 45 days). Hydroxyl free radical (-OH), Malondialdehyde 
  (MDA), Total Antioxidant Capacity (T-AOC), Superoxide Dismutase (SOD) and Catalase 
  (CAT) were measured. The control group&#146; 
  level of -OH and MDA turned higher whereas T-AOC turned lower and SOD and CAT 
  activity elevated after exposed to nitrite solution. The experimental group&#146; 
  level of -OH and MDA turned lower whereas T-AOC turned higher. The results suggested 
  vitamin E supplementation diets could reduce nitrite stress response and increase 
  the antioxidant capacity. The optimal amount of vitamin E supplementation ranged 
  from 180-260 mg kg<SUP>-1</SUP> and number of feeding days ranged from 33-35 
  days were obtained according regress equations.
ER  -