TY  - JOUR
T1  - Effects of Nelin Gene Expression on Phenotypic Switch of Vascular Smooth Muscle Cells
AU - Zhang, Shuguang AU - Pei, Changan AU - Qin, Shiyong AU - Wang, Minghai 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 12
SP  - 706
EP  - 710
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.706.710
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.706.710
KW  - Nelin
KW  -vascular smooth muscle cells
KW  -phenotypic switch
KW  -cytoskeleton
KW  -acetylcholine
AB  - To observe the effects of Nelin gene expression on phenotypic 
  switch of Vascular Smooth Muscle Cells (VSMC). VSMC were induced from synthetic 
  phenotype to contractile phenotype by serum starvation and Nelin mRNA expression 
  levels of different phenotypes of VSMC were checked by using RT-PCR. siRNA was 
  then used to silence Nelin, coomassie blue staining and Acetylcholine (Ach) 
  stimulation were used to observe the changes of VSMC cytoskeleton and contractile 
  functions. Nelin mRNA expression in serum stimulation and serum starvation at 
  24, 48 and 72 h group was 0.1940+0.0971, 0.7114+0.1265, 0.7270+0.1026 and 0.7841+0.1240. 
  The difference was statistically significant (p<0.05). In serum starvation 
  process, VSMC cytoskeleton was reconstructed into uniform, dense bundles from 
  sparse fuzzy networks and regained contractile function. Serum starvation-induced 
  cytoskeletal remodeling was blocked and Ach-induced cell contraction disappeared 
  when Nelin expression was suppressed.
ER  -