TY  - JOUR
T1  - Screening of 11 Pathogens in Fur Animal Basesd on Oligonucleotide Microarray
AU - Xu, Chao AU - Feng, Song-Kai AU - Zhang, Qin AU - Li, Miao-Yun 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 11
SP  - 671
EP  - 677
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.671.677
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.671.677
KW  - microarray
KW  -oligonucleotide
KW  -viruses
KW  -bacteria
KW  -Fur animal
KW  -infection
AB  - Brucella, <I>E. coli</I> O157, <I>Staphylococcus aureus</I>, 
  &#946;-Streptococcus, <I>Erysipelothrix rhusiopathiae</I>, <I>Pseudomonas aeruginosa</I> 
  and Bovine viral diarrhea virus, Sheep pox virus, Goat pox virus, Bluetongue 
  virus, foot and mouth disease virus are common pathogens in fur animal. An oligonucleotide 
  microarray able to detect simultaneously the 6 bacteria and 5 viruses is reported 
  in the present study. The assay was highly specific for detecting the 6 bacteria 
  and 5 viruses in single or multiple infections and as few as 100 copies of specific 
  pathogens target fragments were detected successfully. The 320 archived samples 
  were tested by this assay and the results were 100% consistent with previous 
  results based on conventional PCR and sequencing. The assay is appropriate for 
  the screening of 11 pathogens infections in fur animal due to its high throughput, 
  low-cost, high specificity and sensitivity.
ER  -