TY  - JOUR
T1  - Salvianolate Inhibites Upregulation of TLR4 in Cultured Rat’s Microglia 
  after Oxygen-Glucose Deprivation/Refusion Injury
AU - Wang, Youming 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 11
SP  - 664
EP  - 667
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.664.667
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.664.667
KW  - Salviae miltiorrhizae
KW  -salvianolate
KW  -microglia
KW  -oxygen-glucose deprivation
KW  -TLR4
KW  -RT-QPCR
AB  - To evaluate the effects of salvianolate, an aqueous extract 
  of Salviae miltiorrhizae on the expression of Toll-Like Receptor 4 (TLR4) in 
  microglia after Oxygen-Glucose Deprivation (OGD) followed by refusion using 
  Real Time fluorescence quantitative PCR (RT-qPCR). The expression of TLR4 in 
  primary cultured rat’s microglia was examined after OGD/Refusion injury, 
  the interfering effect of salvianolate on TLR4 expression was investigated under 
  the same condition. Experimental groups; control group 1: the rat<&#146;s microglia 
  cells were maintained in cell culture medium (DMEM/F12 supplemented with 10% 
  heat-inactivated Fetal Bovine Serum (FBS) normal oxygen supply. Model group 
  2: cultured microglia cells were deprived of oxygen-glucose for 4 h and then 
  refused for 12 h. Salvianolate group 3: microglia were incubated with salvianolate 
  dissolved in glucose-free DMEM (10 mg L<SUP>-1</SUP>) during OGD then maintained 
  in the normal cell culture medium containing same concentration of salvianolate 
  during refusion. The expressions of TLR4 in microglia cells in different experimental 
  groups were tested by RT-qPCR. The expression level of TLR4 mRNA in model group 
  was higher than that in control group also higher than that in salvianolate 
  group with statistical significance (p&lt;0.05) however there was no significant 
  difference between the expression in control group and salvianolate group (p&gt;0.05). 
  OGD/Refusion injury activated the TLR4 signaling pathway in cultured microglia. 
  The endogenous mediators might act as the ligands to up-regulate TLR4 and salvianolate 
  significantly inhibited the activity. These results highlighted the importance 
  of salvianolate as a potential therapeutic target in treating cerebral ischemia.
ER  -