TY - JOUR
T1 - Polymorphism of the Keratin 31 Gene in Different Gansu Alpine Fine-Wool Sheep Strains by High Resolution Melting Curve Analysis
AU - Yang, Bihui AU - Niu, Chune AU - Feng, Ruilin AU - , XiaLiang AU - Guo, Jian AU - Sun, Xiaoping AU - Guo, Tingting AU - Yue, Yaojing AU - Liu, Jianbin
JO - Journal of Animal and Veterinary Advances
VL - 13
IS - 10
SP - 627
EP - 632
PY - 2014
DA - 2001/08/19
SN - 1680-5593
DO - javaa.2014.627.632
UR - https://makhillpublications.co/view-article.php?doi=javaa.2014.627.632
KW - analysis
KW -wool traits
KW -high resolution melting
KW -Gansu Alpine fine wool sheep
KW -KRT 31
AB - Keratin Intermediate Filament (KIF) is a key member of the
keratin family of proteins. In this study, High-Resolution Melting (HRM) analysis
and DNA sequencing were used to detect polymorphisms of the Keratin intermediate-filaments
31 (KRT 31) gene in three Gansu Alpine fine wool sheep strains (fine,
super fine and dual purpose strain) (n = 448) and to determine impacts of genotype
for KRT 31 on expression of wool traits. Results showed that there were a total
of two Single Nucleotide Polymorphisms (SNPs) identified as KRT 31 SNP c.166A>G
and c.191T>C. The mutation site c.166 (SNP c.166A>G) results in a missense
mutation from Asn to Ser. The mutation at site c.191 (SNP c.191T>C) does
not alter the amino acid sequence. The χ2-test showed that the
genotype distributions in these three strains of the SNP c.166A>G and SNP
c.191T>C were not all in agreement with Hardy Weinberg equilibrium. Furthermore,
analysis of the impact of KRT 31 gene polymorphism on wool traits in
three Gansu Alpine fine wool sheep strains indicated that the mean fiber diameter
for KRT31c.166A>G genotype AA and AG was smaller than genotype GG (p<0.05)
suggesting this mutation could potentially be exploited in gene marker-assisted
selection programmes within the wool industry to select for animals with decreased
fiber diameter.
ER -