TY  - JOUR
T1  - Complete Genome Sequence of <I>B. abortus</I> RB51 and Genomic Comparison with <I>B. abortus</I> 2308
AU - Chen, Chuangfu AU - Ma, Wolong AU - Guo, Fei AU - Wang, Antao AU - Song, Zhanhui 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 9
SP  - 582
EP  - 588
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.582.588
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.582.588
KW  - B. abortus RB51
KW  -genome sequence
KW  -functional annotation
KW  -wboA gene
KW  -scaffold
AB  - A rifampin-resistant mutant of <I>Brucella abortus</I>, designated as RB51 
  was derived by repeated passage of strain 2308 on Trypticase soy supplemented 
  with 1.5% agar and varying concentrations rifampin or penicillin. In this study, 
  325 Mb data was produced for RB51 using Illumina HiSeq 2000 sequencing platform. 
  Based on the assemble result of RB51, researchers obtained that 36 scaffolds 
  with GC content of 57.25% which contains 150 contigs and has genome size was 
  about 3.26 Mb. Detail bioinformatics annotation of RB51 revealed that the genome 
  contained 3,283 genes, the total length of genes was 2,797,347 bp which makes 
  up 85.91% of genome. Comparative genomics analysis found that there was a tandem 
  repeat sequence of 339 nucleotide in YP_413569.1 protein. In addition, RB51 
  encoded a glycosyltransferase wboA in scaffold12, an enzyme essential in the 
  biosynthesis of O antigen which has been disrupted by IS711. In comparison with 
  the reference genome <I>B. abortus</I> 2308, researchers found that RB51 contained 
  135 SNPs and 5 InDels among which 1 SNP and 2 InDels may be associated with 
  virulent related to lipopolysaccharide.
ER  -