TY  - JOUR
T1  - Apoptosis in the Bovine Oocytes by Different <I>in vitro</I> Culture Conditions
AU - Lim, Hyunjoo AU - Son, Junkyu AU - Yoon, Hobaek AU - Beak, Kwangsoo AU - Park, Sungjai AU - Kwon, Eunggi AU - Chung, Hoyoung 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 9
SP  - 570
EP  - 576
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.570.576
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.570.576
KW  - Apoptosis
KW  -ovaries
KW  -in vitro culture
KW  -milk
KW  -cows
AB  - A total of 88 ovaries in the middle follicular stage were used to detect apoptosis 
  using the modified staining procedure with terminal deoxynucleotide transferase-mediated 
  dUTP nick end-labeling assay (TUNEL), Hematoxylin and Eosin. The analysis observed 
  proportions of cells for apoptosis with several options of culture systems, 
  presenting a control (77.2&plusmn;7.0), culture 1 (3.8&plusmn;2.0), culture 
  2 (1.3&plusmn;2.0), culture 3 (72.7&plusmn;6.0), culture 4 (6.7&plusmn;5.0), 
  culture 5 (80.1&plusmn;6.0) and culture 6 (80.2&plusmn;6.0). The apoptotic cells 
  in cultures 1, 2 and 4 were significantly different with other cultures including 
  the control whereas cultures 3, 5 and 6 showed significantly similar apoptotic 
  patterns with the control. The present analysis observed clear differences for 
  relative numbers of internal ovary cells in apoptosis and necrosis. The new 
  circulation method provided from this study with culture media, levels of gas 
  supply (O<SUB>2</SUB> and CO<SUB>2</SUB>) and incubation temperature for <I>in 
  vitro</I> culture systems can prolong the lifetime of cells.
ER  -