TY  - JOUR
T1  - Construction of a Chimeric Virus Expressing Mutation Sequences of Classical Swine Fever Virus Yunnan Strain
AU - Yang, Yu-Ai AU - Sun, Yong-Ke AU - Zhang, Xiao-Min AU - Du, Min AU - Li, Yu-Xin AU - Kong, Ling-Fu AU - Pan, Hong-Bin AU - Yan, Yu-Lin 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 7
SP  - 470
EP  - 476
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.470.476
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.470.476
KW  - RNA
KW  -CSFV
KW  -chimeric virus
KW  -construction
KW  -genetic stability
KW  -infectious molecular clone
AB  - Infectious cDNA clones are a prerequisite for directed genetic 
  manipulation of RNA viruses. To explore the role of mutations in Classical Swine 
  Fever Virus (CSFV) Yunnan strain which caused the atypical clinical signs in 
  pigs, a new pSM derived from CSFV Shiman strain has been constructed and subsequently 
  replaced by the mutation sequences of CSFV YN strain isolated by the laboratory 
  at the positions 1510-1532, 2471-2658, 3152-3176 and 11785-11816 using the targeted 
  recombination strategy to enable rescue of chimeric CSFV. The results showed 
  that chimeric CSFV (vSM-YN) was successfully rescued from PK-15 cells by transfection 
  of the chimeric CSFV RNA transcripts and identified by whole genome sequence 
  analysis, immunofluorescence antibody assay and ELISA detection. Sequencing 
  of the pAC-SM-YN revealed a high genetic stability and the complete genome sequences 
  of rescued viruses vSM-YN after extensive passages in PK-15 cells showed that 
  modifications in pSM were stably maintained. The results indicate that targeted 
  recombination-mediated mutagenesis provides a powerful tool for expediting the 
  construction of novel RNA genomes and should facilitate further study of the 
  pathogenic mechanism of CSFV leading to atypical CSF.
ER  -