TY  - JOUR
T1  - Development and Evaluation of Vaccines in the Mice Using <I>Brucella suis</I> 
  (<I>B. suis</I>) Deletion Mutant of the <I>vjbR</I> Gene
AU - Chen, Chuangfu AU - Guo, Fei AU - Zhang, Junbo AU - Wang, Yuanzhi AU - Zhang, Hui 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 5
SP  - 306
EP  - 312
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.306.312
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.306.312
KW  - vjbR
KW  -vaccines
KW  -mice
KW  -Brucella suis
KW  -(B. suis)
KW  -gamma interferan
AB  - Brucellosis is an infectious disease that brings great economic burdens for developing countries. The vaccine S2 which is an attenuated <I>Brucella suis</I> (<I>B. suis</I>) strain has been used on a large scale in China. However, the immunity induced by S2 declined relative to those vaccinated with Rev-1 and S19 vaccines. Moreover, the vaccine S2 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent <I>B. suis</I> vaccine is needed. In this study, a vjbR mutant of <I>B. suis</I> (<I>B. suis</I>&#916;vjbR) was constructed overcome these drawbacks. The <I>B. suis</I>&#916;vjbR strain showed reduced survival capability in RAW264.7 macrophage and mice and induced high protective immunity in mice. In addition, <I>B. suis</I>&#916;vjbR induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of gamma interferon (INF&#947;). Further, the vjbR antigen allowed serological differentiation between natural and vaccinated infection in mice. Therefore, <I>B. suis</I>&#916;vjbR was suggested as a safe and efficacious live vaccine candidate against virulent <I>Brucella suis</I> (<I>B. suis</I>) infection.
ER  -