TY  - JOUR
T1  - Effect of &#946;-Carotene on Differential Gene Expression of <I>BCO2</I> 
  and Related Genes During Bovine Adipocytes Differentiation
AU - Lin, Xueyan AU - Wei, Qiaoli AU - Liu, Xiaomu AU - Wan, Fachun AU - Wang, Zhonghua 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 5
SP  - 290
EP  - 296
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.290.296
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.290.296
KW  - B-carotene
KW  -B-carotene-15
KW  -15`-momoxygenase1
KW  -B-carotene-9`
KW  -10`-momoxygenase2
KW  -peroxisome proliferator-activated receptor-
KW  -retinoic acid X receptor a
KW  -bovine adipocytes
AB  - The effects of &#946;-carotene on differentiation of Bovine Intramuscular Preadipocytes (BIP) and Subcutaneous Preadipocytes (BSP) via <I>BCO2</I> and related genes were studied. After treatment of cells with 0, 10, 20, 30, 50 and 80 &#956;M &#946;-carotene, cellular lipid content was assessed by Oil Red O staining and a Triglyceride (TG) assay. The relative expression of &#946;-Carotene-15, 15'-Momoxygenase 1 (BCMO1), &#946;-Carotene-9', 10'-momoxygenase2 (BCO2), Peroxisome Proliferator-Activated Receptor-&#947; (PPAR&#947;) and Retinoic acid X Receptor &#945; (RXR&#945;) were also investigated. Results showed that &#946;-carotene can suppress adipocytes differentiation as a whole. And different concentration &#946;-carotene addition to BSP had not obvious variation of BCO2, PPAR&#947; and RXR&#945; while BIP of same treatment had a highest expression of 30 &#956;M &#946;-carotene at 5 h which can be showed through the results of both Oil Red O staining of cells and fluorescence RT-qPCR. Besides, researchers found that BCMO1 did not nearly express in adipocytes of bovine but BCO2 had a good expression. The present study provides further evidence of the effects of &#946;-carotene on lipid metabolism and suggests that BCO2 may play an important role in the development of bovine adipose tissue <I>in vivo</I>.
ER  -