TY  - JOUR
T1  - Construction of Recombinant pPIC9K-AvBD2 and Expression of Chicken AvBD2 
  Mature Peptide in <I>Pichia pastoris</I>
AU - Qi, Kezong AU - Tu, Jian AU - Xue, Xiuheng AU - Wang, Haixia AU - Wang, Xueyan AU - Peng, Kaisong AU - Xie, Lingling AU - Wu, Yanli 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 4
SP  - 251
EP  - 256
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.251.256
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.251.256
KW  - AvBD2 mature peptide
KW  -P. pastoris
KW  -cloning
KW  -expression
KW  -antibacterial activity
AB  - Avian &#946;-Defensin-2 (AvBD2) is an antimicrobial peptide that plays a significant role in the innate immunity of chickens. In this research, the expression vector pPIC9K-AvBD2 was established and expressed in <I>Pichia pastoris</I>. AvBD2 cDNA was cloned from total RNA of chicken lung by Transcriptase-Polymerase Chain Reaction (RT-PCR). The AvBD2 mature peptide gene was amplified from and cloned into a pPIC9K plasmid. Then the recombinant expression vector pPIC9K-AvBD2 was transformed into <I>P. pastoris</I> GS115 to express the fusion protein. The result showed that a recombinant secretion expression vector pPIC9K-AvBD2 was successfully constructed and a fusion protein about 3.9 kDa chicken AvBD2 was expressed. Densitometric scanning analysis demonstrated that the expressed protein exaccounted for about 63.7% of the total secreted bacterial protein. The expression products had antimicrobial activity to <I>Escherichia coli</I> and <I>Staphylococcus aureus</I> in the Assay of Agar Diffusion Method.
ER  -