TY  - JOUR
T1  - Molecular Characterization and Expression Pattern of a Novel Cadmium/Zinc-Transporting <I>ATPase HMA1</I> Gene
AU - Binwu, Wang AU - Yulong, Gao AU - Zhongbang, Song AU - Wenzheng, Li 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 3
SP  - 159
EP  - 164
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.159.164
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.159.164
KW  - Tobacco
KW  -gene
KW  -cadmium/zinc-transporting ATPase HMA1
KW  -expression pattern
KW  -established
AB  - Cadmium (Cd) of tobacco is a pollutant that is extremely toxic 
  to the health of humans. Cadmium/zinc-transporting <I>ATPase HMA1</I> gene has 
  been characterized to function in the plant cadmium/zinc-transporting. The complete 
  coding sequence of tobacco cadmium/zinc-transporting <I>ATPase HMA1</I> gene 
  was amplified by RT-PCR. The open reading frame of tobacco cadmium/zinc-transporting 
  <I>ATPase HMA1</I> gene was 2418 bp which encodes a protein of 805 amino acids. 
  BLAST analysis revealed that tobacco cadmium/zinc-transporting ATPase HMA1 protein 
  shares high homology with the cadmium/zinc-transporting ATPase HMA1 of potato 
  (85%), <I>Lycopersicon esculentum</I> (84%), wine grape (71%), sweet orange 
  (71%), soybean (69%) and thale cress (68%). Results also showed that tobacco 
  cadmium/zinc-transporting <I>ATPase HMA1</I> gene has a closer genetic relationship 
  with the cadmium/zinc-transporting <I>ATPase HMA1</I> gene of <I>Lycopersicon 
  esculentum</I>. Prediction of transmembrane helices showed that tobacco cadmium/zinc-transporting 
  ATPase HMA1 might be a transmembrane protein. The expression profile was studied 
  and results indicated that tobacco cadmium/zinc-transporting <I>ATPase HMA1</I> 
  gene was moderately expressed in root, leaf and stem but hardly expressed in 
  flower. These results established the primary foundation of utilizing tobacco 
  cadmium/zinc-transporting <I>ATPase HMA1</I> gene to descrease the cadmium content 
  of tobacco and benefit the health of humans in the future.
ER  -